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Résultat majeur : NOVEL INSIGHTS REGARDING THE SIGMOIDAL PATTERN OF RESISTANCE TO NEOMYCIN CONFERRED BY THE APHII GENE IN STREPTOMYCES LIVIDANS.
NOVEL INSIGHTS REGARDING THE SIGMOIDAL PATTERN OF RESISTANCE TO NEOMYCIN CONFERRED BY THE APHII GENE IN STREPTOMYCES LIVIDANS.
02 mai 2013

Nicolas Seghezzi, Marie-Joelle Virolle and Patrick Amar
A library of synthetic promoters of various strengths, specifically constructed for Streptomyces species, was cloned in the promoter-probe plasmid pIJ487, upstream of the promoter-less aphII gene that confers resistance to neomycin. The survival rates conferred by promoters were assessed in the presence of 100 μg/ml neomycin. The correlation between the transcriptional activity of the aphII gene (estimated by RT-PCR) and the resistance to neomycin (expressed as survival rate) indicated a sigmoid rather than a linear correlation. In this issue, we propose a tentative explanation for this sigmoidal pattern of resistance in relation with the level of aphII gene expression. Beyond this specific example, our model might constitute a sound explanation for the generally observed but never explained sigmoidal shape of classical inhibition curves obtained in the presence of linearly increasing antibiotic concentrations.



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